Human TNF-α ELISA Detection Kit


The Human TNF-α ELISA Detection Kit is applicable to the optimization of purification process of biological products, impurity control of intermediate process and release testing of final products. The Human TNF-α ELISA Detection Kit is for research use only and not to be used for diagnostic procedures.

Human TNF-α ELISA Detection Kit

The Human TNF-α ELISA Detection Kit is For Research Use Only

Size: 8 x 12 wells
Sensitivity: 0.21 pg/mL
Dynamic Range: 1.37 – 1000 pg/mL
Sample Size:: 50 uL
Incubation Time: 1 hour 45 minutes
Sample Type: various biological products

Assay Principle

This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Add human TNF-α standard and test samples to the microtiter plate pre-coated with anti-human TNF-α antibody, then add diluted biotin-labeled human TNF-α detection antibody, finally add streptavidin-HRP to form the antibody + antigen + antibody-Biotin + SA-HRP complex, wash the plate and add TMB chromogenic solution for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution. The shade of yellow is positively correlated with the amount of human TNF-α detected in the samples.

Storage Conditions and Shelf Life

The product should be stored and transported at 2-8℃; the shelf life is 12 months.

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Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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