Human TFF2 ELISA Kit

$515.00

The Eagle Biosciences Human TFF2 ELISA Kit is intended for the quantitation of Human TFF2 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human TFF2 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: TF231-K01 Categories: , ,

Human TFF2 ELISA Kit

The Human TFF2 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl
Alternative Name: Trefoil factor 2, SP, SML1


Assay Principle

The Human TFF2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TFF2 with a 96-well strip plate that is pre-coated with antibody specific for TFF2. The detection antibody is a biotinylated antibody specific for TFF2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TFF2 with immunogen: Expression system for standard: NSO; Immunogen sequence: E24-Y129. The kit is analytically validated with ready to use reagents. To measure Human TFF2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human TFF2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human TFF2 in the sample.


Relate Products

Human TFF1 ELISA Kit
Human TFF3 ELISA Kit
Human LIFR ELISA Kit

Additional Information

Assay Background


Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.

Typical Standard Curve


Human TFF2 ELISA Kit

Documents

Product Manual


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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