Human IL-3 ELISA Kit
The Human IL-3 ELISA Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 15 pg/mL
Dynamic Range: 31.25 – 1000 pg/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Names: Interleukin 3, Mast Cell Growth Factor, P-cell stimulating factor, Burst Promoting Activity, Multicolony Stimulating Factor, t h y-1 inducing factor, WEHI-3 growth factor, MULTI-CSF, MCGF, MGC79398, MGC79399
SENSITIVITY, SPECIFICITY AND REPEATABILITY
REPEATABILITY: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
SENSITIVITY: The minimum detectable dose was 15 pg/mL.
SPECIFICITY: This assay recognizes both natural and recombinant human IL-3. The factors listed below were prepared at 10ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Interleukin-3, also known as mast cell growth factor, P-cell stimulating factor, burst promoting activity, multicolony stimulating factor, t h y-1 inducing factor and WEHI-3 growth factor, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. Mature human IL-3 share only 29% aa sequence identity with murine IL-3. IL-3 activity is highly species specific and human IL-3 does not show activity on murine cells. IL-3 is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.