Human IL-10 ELISA Assay
The Human IL-10 ELISA Assay is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 2.44 ng/mL
Dynamic Range: 7.8 – 500 pg/ml
Incubation Time: 3 hours
Sample Type: Serum, Plasma, Cell Culture Supernatant, and other biological fluids
Sample Size: 100 µl
Alternative Names: Interleukin 10, IL10
Assay Background
Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. This cytokine is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production.
Specimen Collection and Handling
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Cell Culture Supernatant: If necessary, centrifuge to remove debris prior to analysis. Samples can be stored at temperature < -20°C. Avoid repeated freeze/thaw cycles.
Serum: Use a serum separator tube and allow clotting for 30 minutes, then centrifuge for 10 minutes at 1000 x g. Remove serum layer and assay immediately or store serum samples at temperature < -20 °C. Avoid repeated freeze/thaw cycles.
Plasma: Collect blood sample in a citrate, heparin or EDTA containing tube. Centrifuge for 10 minutes at 1000 x g within 30 minutes of collection. Assay immediately or store plasma samples at temperature < -20 °C. Avoid repeated freeze/thaw cycles.
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