Human CD124 / IL4-R ELISA Assay Kit
The Human CD124 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 11.5 pg/ml
Dynamic Range: 31.25 pg/ml – 1000 pg/ml
Incubation Time: 1h 45min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Name: IL4-R, Interleukin 4 Receptor
Specimen collection, processing & storage
Cell culture supernatants, serum, plasma or other biological samples will be suitable for use in the assay. Remove serum from the clot or red cells, respectively, as soon as possible after clotting and separation.
Cell culture supernatants: Remove particulates and aggregates by spinning at approximately 1000 x g for 10 min.
Serum: Avoid any unintentional stimulation of the cells by the procedure. Use pyrogen/endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clotting. For that, after clotting, centrifuge at approximately 1000 x g for 10 min and remove serum.
Plasma: EDTA, citrate and heparin plasma can be assayed. Spin samples at 1000 x g for 30 min to remove particulates. Harvest plasma.
Storage: If not analyzed shortly after collection, samples should be aliquoted (250-500μl) to avoid repeated freeze-thaw cycles and stored frozen at –70°C. Avoid multiple freeze-thaw cycles of frozen specimens.
Interleukin 4 or IL-4 is a cytokine whose role is to induce the differentiation of naive helper T cells (Th0 lymphocytes) into Th2 lymphocytes. After being activated by IL-4, Th2 cells themselves start producing IL-4. Their receptor is called the interleukin 4 receptor. The cell that initially produces IL-4 for the differentiation of Th0 has not yet been clearly identified, although recent studies suggest that basophilic polynuclear cells are the effector cells. Interleukin 4 is very close to interleukin 13 which has similar effects.