Human C3 ELISA Kit
The Human C3 ELISA Kit is For Research Use Only
Sizes: 1×96 wells
Sensitivity: 2.524 ng/ml
Standard Range: 12.50 – 400 ng/ml
Incubation Time: 1 hour
Sample Type: Serum and plasma
Sample Size: 100 µl
Product manufactured in the U.S.A.
Assay Principle
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the complement factor 3 (C3) present in samples reacts with the anti-C3 antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-C3 antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C3 in the test sample. The quantity of C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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