Human BPI ELISA Kit

$760.00$1,230.00

The Human BPI ELISA Kit is to be used for the quantitative determination of human BPI in cell culture medium, plasma, wound fluid and bronchoalveolar lavage fluid. The Eagle Biosciences Human BPI ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

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Human BPI ELISA Kit

The Human BPI ELISA Kit is For Research Use Only

Sizes: 1×96 wells and 2×96 wells
Sensitivity: 250 pg/ml
Standard Range: 100-25,000 pg/ml
Incubation Time: 4.5 hours
Sample Type: Cell culture medium, plasma, wound fluid and bronchoalveolar lavage fluid
Sample Size: 100 µl


Assay Background

The antimicrobial protein BPI (Bactericidal Permeability Increasing protein) is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and has also been detected on surface of neutrophils, small intestinal and oral epithelial cells. BPI is a bactericidal compound that is present in polymorphonuclear cells (PMN) and in lower levels in the specific granules of eosinophils. BPI possesses high affinity toward the lipid A region of lipopolysaccharides (LPS) that comprise the outer leaflet of the gram-negative bacterial outer membrane. Binding of BPI to the lipid A moiety of LPS exerts multiple anti-infective activities against gram-negative bacteria: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Airway epithelial cells constitutively express the BPI gene and produce the BPI protein and, therefore, BPI may be a critical determinant in the development of LPS-triggered airway disease. Inflammation induced by LPS possibly contributes to the development of rapid airflow decline, a serious and often fatal complication of hematopoietic cell transplantation.
In plasma of healthy individuals BPI is present at levels of < 0.5 ng/ml, which increases approximately 10-fold during acute phase responses.


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Product manufactured in the U.S.A.

Additional Information

Assay Principle


The human BPI ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human BPI. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human BPI standards (log). The human BPI concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

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