Human Alpha 2-Macroglobulin ELISA


The Human Alpha 2-Macroglobulin ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 2-Macroglobulin in the serum and plasma of humans.

SKU: AME31-K01 Categories: , ,

Human Alpha 2-Macroglobulin ELISA

The Human Alpha 2-Macroglobulin ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 2.409 ng/ml
Standard Range: 20 – 640 ng/ml
Incubation Time: 80 minutes
Sample Type: Biological Fluids
Sample Size: 100 µl
Alternative Names: A2M ELISA,

Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Alpha 2-Macroglobulin present in samples reacts with the anti-Alpha 2-Macroglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A2M in the test sample. The quantity of A2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Specimen Collection and Handling
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles.

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Additional Information

Assay Background

Alpha 2-Macroglobulin (A2M) is a major protease inhibitor in serum and an acute phase protein which increases significantly in concentration in the human as a result of inflammation. The major pathophysiological role for human alpha 2-macroglobulin has yet to be conclusively defined. This kit is specific to the alpha 2- macroglobulin and will not cross react with the closely related alpha 1-macroglobulin.

Typical Standard Curve

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