HLA-C*06:02 (TRATKMQVI) Class I Tetramer

$1,620.00$7,195.00

The HLA-C*06:02 TRATKMQVI Class I Tetramer can be used in flow cytometric analysis to characterise and quantify epitope specific CD8+T cells. The biotinylated monomer can be made into tetramers with optional labels. The Eagle Biosciences HLA-C*06:02 (TRATKMQVI) Class I Tetramer is for research use only and not for diagnostic or therapeutic procedures.

SKU: 1120-02 Categories: ,

HLA-C*06:02 (TRATKMQVI) Class I Tetramer

HLA-C*06:02 TRATKMQVI Class I Tetramer Developed and Manufactured by immunAware

Size: 50, 150, or 500 tests
Organism: HCMV
Epitope: pp65 211-219 (IEDB#: 65997)
HLA: Recombinant HLA-C*06:02(C1G) with a C-terminal biotinylation. The unpaired Cysteine in position 1 is substituted with Glycine.
Formulations: peptide-HLA-I monomer, biotinylated
peptide-HLA-I tetramer with PE or APC label
Buffer: Tris/Maleate pH 7 containing 5% Glycerol
For Research Use Only


Assay Background

This HLA-C*06:02 TRATKMQVI Class I Tetramer protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the β2-microglobulin (β2m) light chain subunit of recombinant HLA class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labelled with PE-conjugated anti-human β2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.


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Recommendation
Please note that the staining intensity can vary between tetramer specificities, hence the tetramer concentration should be titrated the first time a specific tetramer is used. Note, it may be an advantage to stain for the same tetramer specificity with two different fluorochrome labels. It gives a more accurate definition of the tetramer positive population. It also allows for analysis of more than one T cell specificity in the same cell sample. Using various fluorochrome labeled tetramers each specificity can be defined by its unique two fluorochrome combination.

Additional Information

Assay Background


This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the β2-microglobulin (β2m) light chain subunit of recombinant HLA class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labelled with PE-conjugated anti-human β2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.

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