Glutamate ELISA Assay Kit
For Research Use Only
Size: 1×96 wells
Sensitivity: 0.3 µg/ml
Standard Range: 0.6-60 µg/ml
Incubation Time: overnight, 2 x 30 min
Sample Type: Serum, EDTA-Plasma, Urine
Sample Size: 100 µl
After extraction and derivatisation Glutamate is quantitatively determined by ELISA.
The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.