Etanercept (ENBREL) ELISA Assay Kit
The Etanercept (ENBREL) ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 0.1 ug/mL
Standard Range: 0 – 3 ug/mL
Incubation Time: 1 hour 10 minutes
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Name: Enbrel
Assay Background
Etanercept is a protein therapy based on the binding fragment of the tumor necrosis factor alpha receptor used to treat severe rheumatoid arthritis and moderate to severe plaque psoriasis. Etanercept binds specifically to tumor necrosis factor (TNF) and thereby modulates biological processes that are induced or regulated by TNF.7,5 Such processes or molecules affected include the level of adhesion molecules expressed, as well as serum levels of cytokines and matrix metalloproteinases.
Assay Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for etanercept. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to etanercept captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of etanercept in the sample or standard. Results of samples can be determined directly using the standard curve.
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