Equine Anti-SARS-CoV-2 IgG Antibody to spike protein RBD Quantitative TITRATION ELISA

$1,040.00

The Equine Anti-SARS-CoV-2 IgG Antibody to spike protein RBD Quantitative TITRATION ELISA is intended for the Quantitative Estimation of Anti-SARS-CoV-2 IgG antibodies to spike RBD proteins in Equine serum. The Equine Anti-SARS-CoV-2 IgG Antibody to spike protein RBD Quantitative TITRATION ELISA is for research use only and not to be used for diagnostic procedures.

Anti-SARS-CoV-2 IgG Antibody to spike protein RBD Quantitative TITRATION ELISA

The Anti-SARS-CoV-2 IgG Antibody to spike protein RBD Quantitative TITRATION ELISA Assay Kit is For Research Use Only

Size: 12 x 8 wells
Standard Range: 15-100 ng/mL
Incubation Time: 1 hours 15 minutes
Sample Type: Serum
Sample Size: 100 µL


Assay Principle

The method employs indirect sandwich ELISA technique. SARS-CoV-2 Spike RBD-His Recombinant protein is pre-coated onto microwells. Samples and standards are pipetted into microwells and Antibodies to Equine Anti-SARS-CoV-2 (2019-nCoV) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by addition of HRP-conjugated Detection IgG Antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Anti-Equine Anti-SARS-CoV-2 (2019-nCoV) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.


Sample Preparation and Storage

Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.

Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at – 20°C.

Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul sample diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Sample Diluent accordingly.

The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay


Products Related to Anti-SARS-CoV-2 IgG Antibody to spike protein RBD Quantitative TITRATION ELISA

Horse IgG ELISA Assay

Anti-SARS-CoV-2 (COVID-19) IgG Antibody to Spike Protein S1 Quantitative Titration ELISA Assay Kit

Additional Information

Assay Background


The ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. The ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody.

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations