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easYmer H2-Db (Mouse) MHC Tetramers Kit

$1,105.00

easYmer® H2-Db MHC Tetramers Kit can be used to generate monomers with your choice of peptide. The monomers can easily be tetramerized with fluorophore conjugated streptavidin and used to analyze T-cells by flow cytometry. The easYmer reagent can also be used to evaluate specific peptide-HLA I binding. easYmer® H2-Db MHC Tetramers Kit is for research use only.

SKU: 5001-01 Categories: ,

easYmer H2-Db (Mouse) MHC Tetramers Kit

easYmer H2-Db (Mouse) MHC Tetramers Developed and Manufactured by immunAware

Materials Included:

The easYmer kit contains a peptide receptive preparation of HLA, a folding buffer, a positive control peptide for HLA-complex folding. The exact identities of these components are given below:

  • easYmer:H2-Db – peptide receptive, biotinylated in Tris/Maleate pH 7 with 30% Glycerol
  • Folding Buffer: Tris/Maleate pH7
  • Peptide: Influenza NP366-374 (ASNENMETM), a stable HLA-C*07:01 binder. Positive control for evaluation analysis of peptide-HLA folding.

For Research Use Only

 

Available Sizes:

    • 50 Tests (Standard Size)
    • Contact us for bulk orders

Key Benefits of easYmer® MHC Tetramers

  • Ready-to-use
  • One step loading
  • Completely flexible and customizable*
  • Biotinylated
  • No special equipment needed
  • Long shelf-life

*For Custom Tetramer Production, please Contact Us for more information

For Research Use Only
Eagle Biosciences, Inc is an authorized agent for ImmunAware

Assay Principle

This protocol is designed to evaluate the efficiency of peptide- HLA-I interaction and complex formation. The assay is based on detecting the β2-microglobulin (β2m) light chain subunit of recombinant HLA class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labelled with PE-conjugated anti-human β2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub- optimally, or not at all, will give moderate to non-detectable signals.

Related Products

easYmer H2-Db MHC Tetramer Kit
easYmer HLA-A*02:01 MHC Tetramers Kit
easYmer HLA-A*02:03 MHC Tetramers Kit

Additional Information

Assay Background

The easYmer® MHC Tetramers Kit features a highly active formulation of HLA class I (HLA-I) molecules, which can be used to generate specific peptide-HLA class I monomers of your choice in your own laboratory. These monomers can easily be tetramerized with fluorophore conjugated streptavidin and used to stain antigen specific T-cells for analysis in flow cytometric assays. Optionally, the monomers can be stored frozen for later use. The easYmer® technology is highly flexible and suitable for screening of a single epitope in a large number of samples, as well as for screening of a large number of different epitopes in parallel.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations