dsRNA ELISA Assay Kit
The dsRNA ELISA Assay Kit is For Research Use Only
Size: 1 × 96 wells
Sensitivity: unmodified, pUTP modified and N1-Me-pUTP-modified dsRNA: 0.001 pg/μL; 5-OMe-UTP-modified dsRNA: 0.01 pg/μL
Dynamic Range: unmodified, pUTP modified dsRNA: 0 – 1 pg/μL; N1-Me-pUTP-modified dsRNA: 0 – 2 pg/μL; 5-OMe-UTP-modified dsRNA: 0 – 4 pg/μL
Incubation Time: 3 hours
Sample Size: 100 μL
Controls Not Included
Assay Principle
This kit, adopting the principle of double antibody sandwich method and coupling with the streptavidin biotin system, is used for quantitative detection of double stranded RNA (dsRNA) content in samples. The length of dsRNA detected is 60 bp and above, and the dsRNA detected is independent of its nucleic acid sequence. Coat the microtiter plate wells with anti dsRNA antibodies, add samples, incubate and wash, and then incubate with biotinylated detection antibody to form antibody antigen antibody complex, and streptavidin (SA) horseradish peroxidase (HRP) conjugate is added after washing again. After washing again, add streptavidin (SA) horseradish peroxidase (HRP) conjugate. After thorough washing, the TMB substrate is added for chromogenic reaction, and TMB is converted to blue under the catalysis of peroxidase and finally to yellow by the termination effect of acid. The shade of color is positively correlated with dsRNA content in the samples. The absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the dsRNA concentration in the sample is calculated from the standard curve.
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