Denosumab ELISA Assay Kit
The Denosumab ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Sensitivity: < 2.5 ng/mL
Standard Range: 2.5-160 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatants
Sample Size: 100 µL
Alternative Name: Prolia
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Denosumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Denosumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Denosumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Denosumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage for Denosumab Assay:
Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. If samples are to be used for several assays, initially aliquot samples and keep at – 20°C. For Cell Culture Supernatant – If necessary, centrifuge to remove debris prior to analysis. Samples can be stored at -20°C or -80⁰C. Avoid repeated freeze-thaw cycles.