CoproELISA H Pylori Assay Kit
The CoproELISA H Pylori Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: < 2 hours
Sample Type: Stool
Sample Size: 0.1 to 0.3 g
Alternative Name: Heliobacter pylori
H. pylori (previously known as Campylobacter pyloridis) is a type of bacteria that infects the stomach and is a common cause of peptic ulcers. H. pylori bacteria can be passed from person to person through direct contact with saliva, vomit or fecal matter. H. pylori can also be spread through contaminated food or water. The infection is normally acquired during childhood. H. pylori usually goes undiagnosed until symptoms of a peptic ulcer occur. H. pylori infection is quite common and is present in about half the people in the world.
Break-apart microwells are coated with monoclonal anti- H. pylori capture antibodies. Patient samples are diluted in H. pylori Stool Diluent and added to the microwells. A monoclonal antibody conjugated to horseradish peroxidase (HRP) is added to the microwells and incubated for 60 minutes at room-temperature. Unbound conjugate is removed by washing. TMB-substrate is added; the substrate is hydrolyzed by the peroxidase and yields a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm.