C5 ELISA Assay Kit


The Eagle Biosciences Human C5 ELISA Assay Kit is to be used for the quantitative determination of C5 in plasma and serum samples. This Human C5 ELISA Assay Kit is for research use only and not to be used for diagnostic or therapeutic procedures. The analysis should be performed by trained laboratory professionals.

SKU: C5H31 Category:

C5 ELISA Assay Kit

The C5 ELISA Assay Kit is For Research Use Only

Sensitivity: 3.1 ng/mL
Dynamic Range: 3.1 ng/mL – 200 ng/mL
Incubation Time: 3 hours 30 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL
Alternative Names: Complement C5, C5

Assay Background

Complement C5 (molecular weight app. 190kDa) is a key component of the complement system. This system plays important roles in both innate and adaptive immune response and can produce an inflammatory and protective reaction to challenges from pathogens before an adaptive response can occur. The complement system consist of a complex family of proteins and receptors which are found in the circulation, in tissues and other body-fluids. Excessive and uncontrolled activation/regulation of the complement system have been implicated in various diseases such as asthma, lupus erythematosus, glomerulonephritis, various forms of arthritis, autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, paroxysmal nocturnal hemoglobinuria, atypical hemolytic uremic syndrome and ischemia-reperfusion injuries, and rejection of transplanted organs. Three major pathways within the complement system have been described, namely the classical, alternative and lectin pathways which are activated by different stimuli. The classical pathway is initiated by Immune complexes; the lectin pathway by surface bound lectins; and the AP by all the surfaces that are not specifically protected against. All the 3 pathways converge on Complement C3. Cleavage of C3 induces, in turn, the cleavage of C5, successively leading to the production of the membrane attack complex (MAC). This protein complex is the final common effector responsible for lysis of susceptible microorganisms or damage cells. Mutations in C5 gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections.

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Product Manual

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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