Bovine Transferrin ELISA Assay

$460.00

The Bovine Transferrin ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in bovine biological samples.

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Bovine Transferrin ELISA Assay

The Bovine Transferrin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 7.449 ng/ml
Dynamic Range: 18.75 ng/ml – 600 ng/ml
Incubation Time: 60 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL


Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Transferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Transferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Transferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Transferrin in the test sample. The quantity of bovine Transferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


SPECIMEN COLLECTION AND HANDLING
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at ≤-20°C. Avoid repeated freeze-thaw cycles.


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Additional Information

Assay Background


Transferrin is a metal-combining protein that reversibly bound to acid-soluble iron in plasma. It functions to transport iron to the bone marrow and to tissue storage organs such as the liver. Transferrin also participate in the regulation and control of iron absorption and protects against iron intoxication. Like haptoglobin, the carrier of hemoglobin, transferrin is synthesized in the liver, but unlike haptoglobin transferrin is returned to the circulation after unloading its iron in the reticuloendothelial system. This ELISA kit can be used to measure transferrin in serum, tissue extracts and other biological fluids.

Typical Standard Curve


Manual

Product Manual