Big Endothelin-1 ELISA Assay Kit

$580.00

The Eagle Biosciences Human Big Endothelin-1 ELISA Assay Kit is for the determination of Big Endothelin-1 in serum and plasma. The Eagle Biosciences Human Big Endothelin-1 ELISA Assay Kit is manufactured by Biomedica(www.bmgrp.com). The Big Endothelin-1 ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: BI-20082H Categories: , ,

Big Endothelin-1 ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.02 pmol/ml
Dynamic Range: 0.1 – 3 pmol/L
Incubation Time: 5.5 hours
Sample Type: serum, plasma
Sample Size: 50 µL

Controls Included

Product Developed and Manufactured in Austria by Biomedica


Learn more about Endothelin at Eagle Biosciences Biomarker Spotlight page dedicated to Endothelin here: EagleBio Spotlight: Endothelin

Additional Information

Assay Background

Big Endothelin-1 (BigET) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET), represented by amino acids 1-21. ET is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly it has a wide tissue distribution. The cleavage of BigET by Endothelin Converting Enzyme (ECE) leads to ET and to a C-terminal fragment. Both BigET and ET are strong independent predictors of survival in patients with congestive heart failure, and identify a population with a very high risk mortality. The half-life of ET (1-21) in plasma is less than one minute, whereas clearance of BigET is much slower. BigET can therefore be determined more easily.

Assay Principle

  1. All reagents and samples must be at room temperature (18-26°C) before use in the assay.
  2. Mark position for BLANK/STD (Standards)/SAMPLE/CTRL (Control) on the supplied protocol sheet.
  3. Take microtiter strips out of the aluminum bag, take a minimum of one well as Blank. Store unused strips with desiccant at 2-8°C in the aluminum bag. Strips are stable until expiry date stated on the label.
  4. Add 50 µl STD/SAMPLE/CTRL (Standard/Sample/Control) in duplicate into respective well, except blank.
  5. Add 200 µl AB (Detection antibody) into each well, except blank, swirl gently.
  6. Cover tightly and incubate overnight (16-24 hours) at room temperature (18-26°C).
  7. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer), remove remaining WASHBUF by hitting plate against paper towel after the latest wash.
  8. Add 200 µl CONJ (Conjugate) into each well.
  9. Cover tightly and incubate 1 hour at room temperature (18-26°C).
  10. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer), remove remaining WASHBUF by hitting plate against paper towel after the last wash.
  11. Add 200 µl SUB (Substrate) into each well.
  12. Incubate for 30 minutes at room temperature (18-26°C) in the dark.
  13. Add 50 µl STOP (Stop solution) into each well, shake well.
  14. Measure absorbance immediately at 450 nm with reference 630 nm, if available.

Manual

Product Manual


Publications

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