beta Defensin-4 ELISA Assay
The beta Defensin-4 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.8 pg/ml
Dynamic Range: 1.56-100 pg/ml
Incubation Time: 4 hours
Sample Type: Serum, Plasma, Cell culture Supernatants
Sample Size: 100 µL
Specificity: No cross-reactivity was found with the following factors: BD-1 (36 aa), BD-1 (47 aa), BD-2, BD-3.
Intra and inter-assay precision: The CV values of both intra and inter precision fall below 10%.
Alternative Names: BD-4, human B-defensin-4
The beta-Defensin-4 ELISA is for the quantification of BD-4/β-Defensin-4 in serum, plasma and cell culture supernatant. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for BD-4/β-Defensin-4 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any BD-4/β-Defensin-4 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin conjugated antibody specific for BD-4/β-Defensin-4 and the Streptavidin-HRP Enzyme complex are added to each well and incubate. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of BD-4/β-Defensin-4 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450 nm ±2 nm. The concentration of BD4/β-Defensin-4 in the sample is then determined by comparing the O.D of samples to the standard curve.