ASCA IgG ELISA Kit
ASCA IgG ELISA Kit Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: 1 U/ml
Dynamic Range: 1 – 300 U/ml
Incubation Time: 2.5 hours
Sample Type: Serum
Sample Size: 10 µL
Alternative Names: Human ASCA IgG ELISA, ASCA IgG Antibody ELISA
For Research Use Only
The ASCA IgG ELISA Assay Kit is an enzyme immunoassay for the qualitative and quantitative determination of IgG antibodies to Saccharomyces cerevisiae in human serum.
Autoantibodies of the diluted patient samples, calibrators, and the control react with mannan (cell surface component of baker’s yeast) immobilized on the solid phase of a microtiter plate. ASCA IgG guarantees the specific binding of anti-Saccharomyces cerevisiae IgG antibodies of the specimen under investigation by employing purified mannan of Saccharomyces cerevisiae for coating. Following an incubation period of 60 min at room temperature, unbound sample components are removed by a wash step.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish perox-idase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the follow-ing wash step.
HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl¬benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow.
The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Evaluating the test by a semi-quantitative method is also possible.