ARPE-19 Eye Epithelium expressing JRed:
For Research Use Only
A bright red fluorescent cell line has been established by stable transfection of ARPE-19 human retinal pigmented epithelium cells with a plasmid coding for JRed. ARPE-19, a spontaneously arising human RPE cell line can form polarized epithelial monolayers and is a valuable model for epithelial cells.
Cell Line: ARPE-19 cells expressing JRed
Cells/vial: approximately 5 x 105
Subculturing: 1:3 to 1:5
Medium Renewal: 2 to 3 times per week
Growth Medium: 1:1 Ham’s F12/ DMEM (3,1 g/L Glucose) + 10 % fetal calf serum (FCS), 4 mM L-Alanyl-LGlutamine, 2 mM Sodium Pyruvate, 1 % Non Essential Amino Acids (NEA)
Freeze Medium: Complete growth medium + 10% FCS, 10% DMSO, mycoplasma-free
Storage Recommendation: Liquid Nitrogen
Biosafety Level: 1
This product contains a proprietary nucleic acid from coding for a proprietary fluorescent protein(s) intended to be used for research use only.