ANA Screen Quantitative ELISA Assay Kit
ANA Screen Quantitative ELISA Assay Kit Developed and Manufactured Medipan
Size: 12 x 8 wells
Incubation Time: 1 hour 45 minutes
Sample Type: Serum
Sample Size: 10 µL
Alternative Names: Antinuclear Antibody
Controls Included
Assay Background
Autoimmune diseases are based on disorders of the immune system. Synthesized antibodies and autoreactive T cells are directed against endogenous structures and lead to local or systemic inflammatory reactions. In principle, any organ or tissue can be affected by an autoimmune disease. Accordingly, hundreds of autoimmune diseases have been described so far, which can be roughly divided into three groups: In organ-specific autoimmune diseases, individual organs are affected. Systemic, non-organ-specific autoimmune diseases include, for example, collagenosis or other systemic, inflammatory rheumatic diseases. In these cases, antibodies against nuclear or cytoplasmic antigens, which are found in almost all cells in the body, are often detected. In addition, different mixed forms of organ-specific and systemic autoimmune diseases are described.
About 5 to 10 % of the population are affected by an autoimmune disease. The most common are psoriasis, rheumatoid arthritis (RA), diabetes mellitus type 1, multiple sclerosis, Crohn‘s disease and autoimmune thyroid diseases such as Hashimoto‘s thyroiditis and Graves‘ disease. In general, autoimmune diseases are more common in women than in men.
The diagnosis of autoimmune diseases is made on the basis of the clinical symptoms and laboratory medical examinations. The clinical suspicion is confirmed in particular by the detection of antibodies against nuclear or cytoplasmic antigens (ANA) as a characteristic feature in systemic autoimmune diseases such as systemic lupus erythematosus (SLE), Sjögren‘s syndrome, progressive systemic sclerosis (PSS), mixed collagenosis (MCTD), rheumatoid arthritis (RA) or dermatomyositis. The ELISA (Enzyme Linked Immunosorbent Assay) based on a mixture of HeLa cell nuclei, native and recombinant antigens has proven particularly effective for the determination of IgG antibodies. The immunoassay offers the possibility of a sensitive antibody detection.
Assay Principle
The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.
Related Products
ANA Pro (Antinuclear Antibody) ELISA Assay Kit
Anti-Phospholipid Screen ELISA Assay Kit
ANA Screen ELISA Assay Kit
