ANA Pro ELISA Kit
ANA Pro ELISA Kit Developed and Manufactured by Medipan
Size: 1×96 wells
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Antinuclear Antibody Pro ELISA, Human ANA Pro ELISA, Human Antinuclear Antibody Pro ELISA
For Research Use Only
The ANA Pro ELISA Kit is an enzyme immunoassay for the separate semi-quantitative determination of IgG antibodies to nuclear and cytoplasmic antigens.
The antibodies of the calibrator and diluted patient samples react with nuclear and cytoplasmic antigens immobilized on the solid phase of microtiter plates. Highly purified dsDNA, SS-A and Sm as well as recombinant SS-B, RNP (68 kDa, A, C), Scl-70, Jo-1 and CENP-B guarantees the specific binding of autoimmune antibodies of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT), unbound sample components are removed by a wash step.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step.
HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl-benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow.
The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The cut-off is established by multiplying the OD of the calibrator with the respective factor of each antigen of the kit. Patient ratios are calculated by dividing the respective OD of the specimen with the calculated cut-off OD of the respective antigen.