Alpha Synuclein E114C Mutant Monomers: ATTO 488
The Alpha Synuclein E114C Mutant Monomers: ATTO 488 are For Research Use Only
Size: 100 ug
Species: Human
Expression System: E. coli
Buffer: 1X PBS pH7.4
Storage Temperature: -80ºC
Alternative Names: Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson’s disease familial 1 Protein
Product manufactured in Canada by StressMarq.
Scientific Background
The alpha-synuclein (aSyn) E114C mutation facilitates a single site-specific conjugation with ATTO-448 maleimide that avoids any hindrance on fibrilization or cell entry that may be conferred by non-specific lysine targeting conjugations. This conjugation is ideal due to internal position relative to C-terminal truncation sites, proximity to the NAC, and lack of interference with recruitment in vitro or in primary neurons. Pre-formed fibrils (PFFs) generated with 5-25% fluorescently tagged E114C mutants have demonstrated a relative potency >80% compared to wild-type aSyn for inducing misfolding of endogenous aSyn, indicating no significant perturbation of seeding in living cells. Atto-488 is a useful tool for identifying cell entry, as the addition of Trypan Blue to cultures prior to imaging will quench fluorescence of extracellular Atto-488 conjugated aSyn. Our aSyn E114C-Atto-488 PFFs, which are formed from 10% fluorescently tagged E114C mutants and 90% wild-type monomers, are an excellent tool for studying cell entry and localization, with demonstrated entry into neurons after trypan blue quenching.
