Adalimumab (HUMIRA) ELISA Assay Kit
The Adalimumab (HUMIRA) ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 10 ng/mL
Standard Range: 0 – 1000 ng/ml
Incubation Time: 1 hour 10 minutes
Sample Type: Serum, Plasma
Sample Size: 20 µL
Alternative Name: Humira
Assay Background for Adalimumab Assay
Adalimumab binds with specificity to tumor necrosis factor-alpha {TNF-alpha) and inhibits its interaction with the pSS and p75 cell surface TNF receptors. Adalimumab also lyses surface tumor necrosis factor expressing cells in vitro when in the presence of complement. Adalimumab does not bind or inactivate lymphotoxin {Tumor necrosis factor- beta). TNF is a naturally occurring cytokine that plays a role in normal inflammatory and immune responses. Increased levels of TNF are found in the joint synovial fluid of rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis patients, and play an imperative role in pathologic inflammation and the joint destruction that are major complications of these diseases. Increased levels of TNF are also measured in psoriasis plaques. In plaque psoriasis, treatment with adalimumab may decrease the epidermal thickness and inflammatory cell infiltration. The relationship between this pharmacodynamics and the mechanism{s) by which adalimumab achieves its clinical effects is not known. Additionally, adalimumab alters biological responses that are induced/regulated by TNF, including changes in the levels of adhesion molecules responsible for leukocyte migration during inflammation {ELAM-1, VCAM-1, and ICAM-1 with an ICSO of 1-2 X 10-lOM).
Assay Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for adalimumab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to adalimumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of adalimumab in the sample or standard. Results of samples can be determined directly using the standard curve.
