HeLa human carcinoma expressing TurboGFP
The HeLa human carcinoma expressing TurboGFP is For Research Use Only
Human cervix carcinoma cells were stably transfected with a plasmid coding for pTurboGFP resulting in a bright green fluorescent cell line.The HeLa cell line was established as the first cell line from a cervix carcinoma of a 31-year-old woman in 1951. These cells proliferate rapidly and display an active telomerase enzyme avoiding normal aging and cell death through shortening of the telomeres. HeLa cells are very durable, easy to handle and widely used in cell culture-based research.
Specifications
Cell Line: HeLa cells expressing TurboGFP
Cells/vial: approximately 5 x 105
Subculturing: 1:3 to 1:5
Medium Renewal: 2 to 3 times per week
Growth Medium: DMEM (1 g/L D-Glucose, 3,7 g/L NaHCO3) + 10 % fetal calf serum (FCS), 2 mM L-Alanyl-LGlutamine, 1 % Non Essential Amino Acids (NEA)
Freeze Medium: Complete growth medium + 10% FCS, 10% DMSO
Shipping: Frozen
Storage Recommendation: Liquid Nitrogen
Biosafety Level: 1
INFORMATION:
Organism: homo sapiens (human)
Source: Organ: cervix, disease: adenocarcinoma
Gender: female, 31 years
Ethnicity: African American
Growth properties: adherent
Morphology: epithelial
Conditions: 37 °C, 5 % CO2
Plasmid: pTurboGFP (Evrogen, Moscow, Russia)
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