Bovine IgG1 ELISA Assay
The Bovine IgG1 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 78 ng/ml (sample dilution 1:10)
Dynamic Range: 7.8 – 500 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Biological Fluids
Sample Size: 10 µL
Alternative Name: Cow IgG1 ELISA Assay
Control Included
Assay Principle
The determination of bovine IgG1 is carried out as direct sandwich ELISA. An antibody specific for bovine IgG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG1 present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG1 concentration.
