Plasma Metanephrine ELISA Assay Kit
For Research Use Only
Size: 1×96 wells
Sensitivity: 14.9 pg/ml
Standard Range: 36 – 3 600 pg/ml
Incubation Time: 5 hours 25 min
Sample Type: Plasma
Sample Size: 200 µl
Controls Included
Assay Principle
Metanephrine (Metadrenaline) is first extracted using an ion exchange matrix followed by an acylation process. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
