Anti-Semaglutide (OZEMPIC) Quantitative ELISA Assay Kit
The Anti-Semaglutide (OZEMPIC) Quantitative ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 5 ng/mL
Standard Range: 0 – 150 ng/mL
Incubation Time: 2 hour 20 minutes
Sample Type: Serum, Plasma
Sample Size: 25 µL
Alternative Name: Ozempic
Controls Included
Assay Background
Semaglutide is a GLP-1 analogue with 94% sequence homology to human GLP-1. Semaglutide acts as a GLP-1 receptor agonist that selectively binds to and activates the GLP-1 receptor, the target for native GLP-1. GLP-1 is a physiological hormone that has multiple actions on glucose, mediated by the GLP-1 receptors. The principal mechanism of protraction resulting in the long half-life of semaglutide is albumin binding, which results in decreased renal clearance and protection from metabolic degradation. Furthermore, semaglutide is stabilized against degradation by the DPP-4 enzyme. Semaglutide reduces blood glucose through a mechanism where it stimulates insulin secretion and lowers glucagon secretion, both in a glucose-dependent manner. Thus, when blood glucose is high, insulin secretion is stimulated, and glucagon secretion is inhibited. The mechanism of blood glucose lowering also involves a minor delay in gastric emptying in the early postprandial phase.
Assay Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the drug semaglutide. After incubation, the wells are washed. Then, Horse Radish Peroxidase (HRP) conjugated probe is added and binds to semaglutide antibodies captured by the drug semaglutide on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of semaglutide antibodies in the sample or standard. Results of samples can be determined directly using the standard curve.
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