CH50 ELISA Assay Kit
The CH50 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Incubation Time: 2 hours 15 minutes
Sample Type: Serum (pre-treatment required)
Sample Size: 50 µL
Controls Included
Assay Background
The primary utility of the CH50 in the practice of an allergist-immunologist is to screen for complement deficiency associated immunodeficiency (primarily classic or terminal complement component deficiencies). Absent or significantly reduced individual complement components may result in infections, neisserial meningitis, or sepsis. A reduced CH50 in this situation warrants quantification and functional assays of individual complement components. Reduction of the CH50 occurs when individual complement component(s) are deficient or consumed.
Assay Principle
The complex β-galactosidase/anti-β-galactosidase, is solubilized by serum through the deposition of C3b molecules. The formation of C3b quantity necessary for the solubilization is mediated by alternative pathway, but it is accelerated from activity of C3-convertasi by classic way. The quantity of complex β-galactosidase/Anti β-galactosidase dissociated from the antibody, detectable by enzymatic activity in the supernatant at the end of the reaction, is a measure of serum capacity to form C3b molecules. The o-nitrophenilgalactopiranoside (o-NPG) is used as substrate and the measure of reagent product (onitrophenol) is read at 420nm (or 405 nm).
