Tuna IGF-I (Thunnus maccoyii)
The Eagle Bioscience’s Tuna IGF-I (Thunnus maccoyii) is for research use only and not to be used for diagnostic procedures.
IGF-I is produced in E.coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.
Tuna insulin-like growth factor-I (IGF-I) is a single chain 68 amino acid polypeptide that shares structural homology with human IGF-I. Seventeen amino acids are different compared to the sequence of human IGF-I. Three of these changes occur in the B-domain, seven in the variable C-domain, two in the A-domain and five in the D-domain. Tuna IGF-I can be used for a variety of in vivo and in vitro applications including those investigating the actions of growth hormone.
- Purity: >95 % (as determined by HPLC analysis)
- Quantity: 100 µg, 5×100 µg
- Molecular Weight: 7481 daltons – confirmed by Mass Spectrometry
- N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
- Biological Activity: Stimulation of protein synthesis in rat L6 myoblasts (ED50 < 50 ng/ml)
- Endotoxin (LAL): <0.1 EU/ug
- Storage/Stability: At least 2 years at 2-4°C (lyophilized)
- Appearance: White Powder
- State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)