RevertUP II Reverse Transcriptase
The RevertUP Reverse Transcriptase is a DNA polymerase which uses RNA as a substrate and exhibits no measurable proofreading 3’→5′ exonuclease function. This enzyme performs cDNA synthesis by extending a DNA primer annealed to an RNA template; it can also make copies of single-stranded DNA templates. The RevertUP II Reverse Transcriptase enzyme has no RNase H activity and ensures efficient synthesis of ≥19 kb cDNA.
Unit Definition: One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble fraction in 10 minutes at 42°C when poly(A)+ RNA and oligo(dT)20 are used as template–primer.
This RevertUP II Reverse Transcriptase is manufactured in Germany by biotechrabbit.
