AllScript Reverse Transcriptase

AllScript Reverse Transcriptase

Price range: $270.00 through $1,210.00

The AllScript Reverse Transcriptase (4 U/µl) is a proprietary RT designed for highly specific and sensitive reverse transcription. It performs standard reverse transcription, synthesis of ds cDNA for cloning, RT-PCR and qRT-PCR, rapid amplification of cDNA ends (RACE) or RNA analysis by primer extension. The AllScript Reverse Transcriptase (4 U/µl) is for research use only and not to be used for diagnostic procedures.

SKU: BR040060 Category: Tags: ,

AllScript Reverse Transcriptase

AllScript Reverse Transcriptase is a multifunctional enzyme including RNA-dependent and ssDNA-dependent DNA polymerase, as well as RNase H activity. The RNase H activity is specific to RNA hybridized to cDNA, with no effect on pure RNA template, resulting in improved performance of subsequent PCR.
Unit Definition: One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble fraction in 10 minutes at 42°C when poly(A)+ RNA and oligo(dT)20 are used as template–primer.

This AllScript Reverse Transcriptase is manufactured in Germany by biotechrabbit.

Size 400 units | 2,000 units
Component
  • AllScript Reverse Transcriptase – AllScript Reverse Transcriptase, 4 U/µl, in storage buffer containing 50% (v/v) glycerol.
  • 5× Reverse Transcriptase Buffer – Optimized 5× Reaction Buffer for AllScript Reverse Transcriptase.
Application Standard reverse transcription, Synthesis of ds cDNA for cloning, RT-PCR and qRT-PCR, Rapid amplification of cDNA ends (RACE), RNA analysis by primer extension
Hot Start No
Storage -20°C

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