Anti-Beta2-Glycoprotein 1 IgG ELISA Kit
Anti-Beta2-Glycoprotein-1 IgG ELISA Kit Developed and Manufactured by Svar Life Science
Size: 1×96 wells
Sensitivity: See Package Insert
Dynamic Range: Cut-off
Incubation Time: 2 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Anti-Beta 2 GP1 IgG ELISA, Human Anti-Beta 2 Glycoprotein 1 IgG ELISA,
For Research Use Only
Sample Collection and Storage: The assay is recommended for serum/plasma (citrate or EDTA anticoagulated) samples; do not use lipaemic, haemolysed, icteric or turbid samples. Thoroughly mix thawed samples before assay and avoid repeated freeze/thawing. Do not heat-inactivate samples, this may yield false positive results. Samples may be stored undiluted or at 1:101 dilution in diluted Sample Diluent 2 at -20° C or 2-8° C for four weeks.
<10U/mL = Negative ≥10-15U/mL = Equivocal Result >15U/mL = Positive
It is recommended that each laboratory establish their own normal range.
In this Anti-Beta2-Glycoprotein-1 IgG ELISA Kit, wells of the microtitre strips are coated with highly purified human β2GP1 antigen. During the first incubation, specific autoantibodies in diluted serum or plasma bind to the antigen-coated surface. The wells are then washed to remove unbound components. In the second incubation, the Conjugate, enzyme-labeled antibodies to human IgG, binds any surface-bound autoantibodies. After further washing, specific autoantibodies are traced by incubation with the Substrate. Addition of Stop Solution terminates the reaction, resulting in a colored end-product. The amount of Conjugate bound is measured in absorbance units. In the qualitative protocol, the amount of Conjugate bound by the sample is compared with that bound by the Reference Control. In the quantitative protocol, the concentration of anti-β2GP1 autoantibody can be estimated by interpolation from a dose-response curve based on Standards formulated from a high titer plasma and assigned arbitrary units.