GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.
The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Exonuclease 1 (ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products. GenUP Exo SAP Kit is for research use only and not to be used for diagnostic procedures.
Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3′ → 5′ exonuclease activity of Exonuclease 1 degrades single-stranded primers.
This GenUP Exo SAP Kit is manufactured in Germany by biotechrabbit.
