Neutrophil Elastase, a major serine proteinase in human, is predominantly present in the
azurophilic granules of neutrophils. Elastase has a broad range of extracellular matrix
substrates including elastin, proteoglycans, collagen and fibronectin. The action of Elastase is
controlled by serine proteinase inhibitors. Elastase, when released during inflammation, is
rapidly bound by its two main inhibitors, alpha1-PI and alpha2-macroglobuline to form
Elastase-inhibitor complexes. In addition, mucosa secretions may contain the locally secreted
Elastase inhibitors elafin/SKALP and SLPI.
When secreted at sites of inflammation Elastase can cause severe tissue damage. An
important role has been suggested for Elastase in various inflammatory disorders, including
pulmonary emphysema, sepsis, arthritis, nephritis and certain skin diseases. Elastase induces
the production of IL-8 in human bronchial epithelial, a proces that occurs in part through TLR4.
In plasma of healthy individuals approx. 160 ng/ml (30 – 350 ng/ml) Elastase is present.
This product is manufactured in Netherlands by Hycult Biotech.
| Size | 1 x 96 Well |
| Sensitivity | 0.4 ng/mL |
| Dynamic Range | 0.4 – 25 ng/mL |
| Incubation Time | 3 hours 30 minutes |
| Sample Type | Plasma, Cell Culture Supernatant |
| Storage | 2-8°C |
| Alternative Names | Neurophil Elastase |