SpheroTribe
Kit Description:
The full kit includes the required SpheroTribe solution, U-bottom plates, and wide-opening tips. Everything you need to get started with your 3D cell culture experiments.
- 2.5 mL Kit (96 spheroids):
- 2.5mL of 5X methylcellulose solution
- 1x U-bottom 96-well plate
- 20 pipette tips (200µL) with a large opening
- 25 mL Kit (960 spheroids):
- 25 mL of 5X methylcellulose solution
- 10x U-bottom 96-well plates
- 2x racks of 96 pipette tips (200µL) with a large opening
*Contact us us if you require just the methylcellulose solution
Features:
- Cell aggregation booster: SpheroTribe provides a gel-like scaffold that favors cell-cell contacts by increasing medium viscosity. It was shown to generate compact spheroids mimicking solid tumors, improve spheroid formation with some of the most challenging cells and to speed up stem cell-derived organoid formation.
- Increased homogeneity: By maximizing cell aggregation, SpheroTribe promotes the formation of unique & uniformly sized spheroids allowing for consistent assays (growth, invasion, immune infiltration, in vivo injection, etc).
- Easy to use: No need to work on ice or have access to sophisticated equipment. With SpheroTribe, you have everything on hand to easily grow & handle your spheroids.
- Universal: The SpheroTribe solution is composed of methylcellulose, a biologically inert compound dissolved in basal culture medium without any proteins, lipids or growth factors. You can dilute it in any culture medium of your choice, and add additional compounds as desired (i.e. serum, antibiotics, differentiation factors, etc).
Applications:
- SpheroTribe has been successfully used for spheroid/organoid formation with the following cell types:
- Patient-derived stem-like glioblastoma cells (GB P3, BL13)
- Human glioblastoma cell lines (U87 & T98G)
- Human osteosarcoma cell lines (U2-OS, Saos-2)
- Human neuroblastoma cell lines (NB-EBc1 and SK-N-AS)
- Human vaginal mucosal melanoma (HMV-II)
- Human primary colorectal cancer cells
- Human colorectal cancer cells (HT-29 & HCT-116)
- Human breast cancer cells (MDA-MB 231)
- Human induced pluripotent stem cells (iPSC)
- HeLa cell line
- Monkey kidney fibroblast-like cell line (COS-7)
- Primary neurons from rat embryos (E18)
- Murine melanoma cells (B16F10)
- Murine lung adenocarcinoma cells (KP-HELLO-2)
- Experimental Assays: Once spheroids have grown to your desired size, you can use them for any kind of assay according to your regular workflow. The SpheroTribe solution can be readily washed off, leaving a spheroid available for other tests at any stage of your protocol.
- Example of in situ assays you can perform directly on the U-bottom plate supplied:
- Live imaging
- Growth/proliferation studies
- Toxicity studies
- Examples of downstream assays that might require transferring spheroids to other vessels:
- Invasion & migration assays
- Immunostaining
- Biochemical assays
- Immune infiltration assays
- In vivo implantation
This SpheroTribe is manufactured in France by Idylle SAS.
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| Storage | 2-8°C |
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