RevertUP II Reverse Transcriptase

RevertUP II Reverse Transcriptase

Price range: $135.00 through $540.00

The RevertUP II Reverse Transcriptase (200 U/µl) is a proprietary MMuLV reverse transcriptase engineered by point mutations resulting in increased temperature stability up to 60°C. The enzyme has no RNase H activity and ensures efficient synthesis of ≥19 kb cDNA. The RevertUP II Reverse Transcriptase (200 U/µl) is for research use only and not to be used for diagnostic procedures.

SKU: BR040050 Category: Tags: ,

RevertUP II Reverse Transcriptase

RevertUP Reverse Transcriptase is a DNA polymerase which uses RNA as a substrate and exhibits no measurable proofreading 3’→5′ exonuclease function. This enzyme performs cDNA synthesis by extending a DNA primer annealed to an RNA template; it can also make copies of single-stranded DNA templates.
Unit Definition: One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble fraction in 10 minutes at 42°C when poly(A)+ RNA and oligo(dT)20 are used as template–primer.

This RevertUP II Reverse Transcriptase is manufactured in Germany by biotechrabbit.

Size 10,000 units | 50,000 units
Component
  • RevertUP II Reverse Transcriptase – RevertUP II Reverse Transcriptase, 200 U/µl, in storage buffer containing 50% (v/v) glycerol.
  • 5× RevertUP II Buffer – Optimized 5× RevertUP II Reverse Transcriptase reaction buffer.
Application First-strand cDNA synthesis, Generation of labeled cDNA, RNA analysis by primer extension, cDNA library construction, RT-PCR
Hot Start No
Storage -20°C

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