NZY RNase H (E. coli)
NZY RNase H is a recombinant endoribonuclease purified from an Escherichia coli strain that over-expresses the cloned RNase H gene (rnh). The enzyme specifically hydrolyses the phosphodiester bonds of RNA which is hybridized to DNA to produce 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. RNase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as cDNA-RNA hybrids in the amplification reaction may prevent binding of the amplification primers. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets.
This NZY RNase H (E. coli) is manufactured in Portugal by NZYtech.
| Size | 250 U|1250 U |
| Component |
|
| Application | cDNA synthesis, RT-PCR, RT-qPCR |
| Hot Start | No |
| Storage | -20°C |
