Mouse Immunoglobulin M (IgM) is the earliest antibody isotype produced during an immune response and plays a vital role in the primary defense mechanism against pathogens. Structurally, IgM exists mainly as a pentamer, giving it high avidity for antigens and strong complement-activating potential. Because it is the first antibody produced before isotype switching to IgG, IgM serves as a key indicator of early-stage immune activation. The Mouse IgM ELISA (Enzyme-Linked Immunosorbent Assay) is a quantitative assay used to measure total or antigen-specific IgM levels in biological samples such as serum, plasma, or cell culture supernatants. It employs antibodies specific for mouse IgM and an enzyme-linked detection system that produces a measurable colorimetric signal proportional to IgM concentration.
In research, the Mouse IgM ELISA is a fundamental tool for studying primary humoral immune responses in vaccination, infection, and autoimmune models. It allows researchers to track the onset and magnitude of immune activation following antigen exposure, distinguishing early IgM production from later IgG responses. The assay is also used in B-cell development studies, monoclonal antibody generation, and immunotoxicology to assess immune competence or dysfunction in experimental mice. Because IgM reflects initial antigen recognition, its measurement provides valuable insights into immune system activation dynamics and the effectiveness of novel immunogens or adjuvants.
While primarily used in preclinical research, Mouse IgM ELISA data have strong translational significance. Mouse IgM responses closely model human immune kinetics, making the assay valuable in vaccine development, infectious disease studies, and autoimmune research. Monitoring IgM levels can help differentiate acute versus chronic immune responses, an important principle also used in clinical serology. In summary, the Mouse IgM ELISA is a quantitative biomarker assay that enables precise assessment of early antibody-mediated immunity, serving as a critical bridge between preclinical immunological research and clinical diagnostics or therapeutic innovation.
This product is manufactured in USA by Eagle Biosciences.