Human Insulin Receptor CT-1 (C Terminal) Monoclonal Antibody
The Eagle Bioscience’s Human Insulin Receptor CT-1 (C Terminal) Monoclonal Antibody is for research use only and not to be used for diagnostic procedures.
Produced in hybridoma cells derived from the NS-1 myeloma cell line. Purified from conditioned medium by Protein A affinity chromatography
Human insulin receptor antibody CT-1 is a mouse monoclonal antibody (IgG1) that recognizes an epitope within amino acids 1337-1355 in the C-terminal domain of the human insulin receptor. The antibody was prepared against a C-terminal peptide sequence of the human insulin receptor β-subunit (KKNGRILTLPRSNPS). The antibody reacts with native human and rat insulin receptors in solution, whether or not insulin is bound and whether or not the receptor has undergone prior tyrosine autophosphorylation.
- Quantity: 200 µg
- Immunogen: The hexadecapeptide YKKNGRILTLPRSNPS conjugated to keyhole limpet haemocyanin or bovine serum albumin
- Specificity: Cross-reacts with the human and bovine TGF-β2. It does not bind to human TGF-β1 or TGF-β3
- Appearance: Lyophilized white powder
- Storage: At least 2 years at 2 – 8°C (lyophilized). After reconstitution store at -20°C or -80°C. Avoid freeze-thaw cycles
- Reconstitution: Dissolve in 200 μl phosphate buffered saline pH 7.4
- Application and Titre: Each application and titre should be determined in house but as a guideline: Western immunoblotting (1:5,000); Immunoprecipitation (1:5,000); Enzyme immunoassay (1:5,000)