Human Insulin Receptor 83-7 (Alpha Chain) Monoclonal Antibody
The Eagle Bioscience’s Human Insulin Receptor 83-7 (Alpha Chain) Monoclonal Antibody is for research use only and not to be used for diagnostic procedures.
Produced in hybridoma cells derived from the NS-1 myeloma cell line. Purified from conditioned medium by Protein A affinity chromatography.
Human insulin receptor antibody 83-7 is a murine monoclonal antibody (IgG1) that recognizes an epitope within amino acids 140-301 (the cysteine rich region) of the extracellular domain of the human insulin receptor. The antibody enhances the binding of insulin to the insulin receptor of 3T3 cells and stimulates insulin-mediated thymidine incorporation in these cells.
- Quantity: 200 µg
- Immunogen: IM9 lymphocyte preparation
- Specificity: Cross-reacts with the human insulin receptor but not the rat insulin receptor or the human type 1 IGF receptor
- Appearance: Lyophilized white powder
- Storage: At least 2 years at 2 – 8°C (lyophilized). After reconstitution store at -20°C or -80°C. Avoid freeze-thaw cycles
- Reconstitution: Dissolve in 200 μl phosphate buffered saline pH 7.4
- Application and Titre: Each application and titre should be determined in house but as a guideline: Western immunoblotting (1:5,000); Immunoprecipitation (1:5,000); Enzyme immunoassay (1:5,000)