Human Insulin Receptor 83-14 (Alpha Chain) Monoclonal Antibody

$460.00

The Eagle Bioscience’s Human Insulin Receptor 83-14 (Alpha Chain) Monoclonal Antibody is for research use only and not to be used for diagnostic procedures.

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Human Insulin Receptor 83-14 (Alpha Chain) Monoclonal Antibody

The Eagle Bioscience’s Human Insulin Receptor 83-14 (Alpha Chain) Monoclonal Antibody is for research use only and not to be used for diagnostic procedures.


Product Specifications

Source:

Produced in hybridoma cells derived from the NS-1 myeloma cell line. Purified from conditioned medium by Protein A affinity chromatography 

Description:

Human insulin receptor antibody 83-14 is a mouse monoclonal antibody (IgG2a) that recognizes an epitope within amino acids 469-572 in the extracellular domain of the human insulin receptor. The antibody was prepared by immunising mice with a preparation of IM9 lymphocytes and then with purified insulin receptor. The antibody strongly inhibits the binding of insulin to the insulin receptor in 3T3 cells. 

  • Quantity: 200 µg
  • Immunogen: IM9 lymphocyte preparation
  • Specificity: Cross-reacts with the human insulin receptor but not the rat insulin receptor or the human type 1 IGF receptor
  • Appearance: Lyophilized white powder
  • Storage: At least 2 years at 2 – 8°C (lyophilized). After reconstitution store at -20°C or -80°C. Avoid freeze-thaw cycles
  • Reconstitution: Dissolve in 200 μl phosphate buffered saline pH 7.4
  • Application and Titre: Each application and titre should be determined in house but as a guideline: Western immunoblotting (1:5,000); Immunoprecipitation (1:5,000);  Enzyme immunoassay (1:5,000)

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Package Inserts

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations