Human CD124 / IL4-R ELISA Assay Kit

$490.00

The Eagle Biosciences Human IL-4R ELISA is to be used for the in-vitro quantitative determination of soluble IL-4 receptor molecule (CD124)  in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL-4R. The Eagle Biosciences Human IL-4R ELISA Assay kit is for Research Use Only.

SKU: C2431-K01 Categories: , ,

Human CD124 / IL4-R ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 11.5 pg/ml
Dynamic Range: 31.25 pg/ml – 1000 pg/ml
Incubation Time: 1h 45min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl

Product manufactured in France by Diaclone

Additional Information

Assay Principle


The IL-4R Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations and unknowns are pipetted into these wells.  During the first incubation, the IL-4R antigen and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated.  After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-4R present in the samples.

  1. Prepare Standard curve as shown in section 8.4
  2. Add 100µl of each standard, sample, control and zero (standard diluent) in duplicate to appropriate number of wells
  3. Add 50µl of diluted biotinylated anti-IL-4R to all wells
  4. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 1 hours
  5. Remove the cover and wash the plate
  6. Add 100μl of Streptavidin-HRP solution into all wells
  7. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min
  8. Repeat wash step 5.
  9. Add 100μl of ready-to-use TMB Substrate Solution into all wells
  10. Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil
  11. Add 100μl of H2SO4:Stop Reagent into all wells

Typical Standard Curve


Manual

Product Manual