Heat Labile Uracil-DNA Glycosylase, 1 U/µL

Heat Labile Uracil-DNA Glycosylase, 1 U/µL

Heat Labile Uracil–DNA Glycosylase is completely and irreversibly inactivated by moderate heat treatment at 50⁰C, allowing contamination control in RT-qPCR. The enzyme hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic (apyrimidinic) site in DNA but does not modify uracils in RNA. Heat Labile Uracil–DNA Glycosylase is highly active at 20–40°C. No cofactors or divalent cations are required for activity, and the enzyme is active in most PCR and RT-PCR buffers. Although the enzyme is active a pH 6.5–9.0, the optimal pH 7.5 is in 50 mM NaCl.
Unit Definition: One Unit will liberate 1 nmol Uracil from Uracil-containing DNA per hour at 37°C.

This Heat Labile Uracil-DNA Glycosylase, 1 U/µL is manufactured in Germany by biotechrabbit.