FakirSlide Coverslip
Kit Contents
- Ready-to-use FakirSlide structured coverslips with your choice of nanostructure(s)
- Stencells (1 per coverslip) for optimized coating & multiplied experimental conditions, with your choice of design(s)
- 1. Select your favorite structures: Choose your coverslip(s) among our available catalog, according to your preferred design(s).
- Round pillars (small, large)
- Square pillars
- Nanodomes (small, large)
- Nanocones
- 2. Add some Stencells to your order at no extra cost. Stencells are silicon chambers that you can easily stick and remove from the FakirSlide coverslips to create controlled culture areas. They will help you make the most of your FakirSlide coverslips.
- Optimize your coating: get a homogeneous coating while saving reagents by using the Solo, Quartet or Nonet Stencells.
- Increase your outputs: don’t need a large surface? Use the Quartet & Nonet designs to multiply your experimental conditions on a single FakirSlide coverslip and create structure-free control areas.
Features:
- Unlock new insights: Based on a newly-developed nanostructuration technique called soft nanoimprint lithography, FakirSlide pushes the resolution boundaries and makes available to researchers a diverse panel of reproducible micro to nano-scale topographies of various aspect ratios to better investigate key cellular responses to specific membrane topologies. Check out our available catalog below.
- Maximize your assay reliability: FakirSlide provides ordered arrays of structures with high control over their shape, diameter and periodicity. They were specifically designed to induce robust & consistent membrane curvatures and facilitate data analysis.
- Simplify your experimental workflows: made of a high-quality synthetic silica layer, the Fakirslide surface is directly functional for supported lipid bilayers without the need for harsh cleaning or hydroxylating pre-treatments.
Fakirslides substrates have been successfully used to manipulate membrane morphology of living cells and supported lipid bilayers, and observe effects of curvatures on membrane protein dynamics, cytoskeletal reorganization and cell migration. Check out relevant publications and example results in our dedicated sections.
Applications:
- Use FakirSlide to apply membrane deformations on cultured cells or membrane-mimetic systems. Experimental outputs include:
- Live cell imaging
- Immunostaining
- Migration assays
- Compatible imaging modes: confocal microscopy, Airyscan microscopy, TIRF, super-resolution microscopy (2D and 3D STED, PALM/STORM), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM).
- Cell types: So far, FakirSlide has been successfully used with a variety of human (HeLa, U-2 OS, HT1080, SUM159, RPE-1, THP-1 & human monocyte-derived dendritic cells (moDC)) and murine (C2C12 mouse myoblasts) cells.
- Supported membranes: So far, FakirSlide has been successfully used with a variety of neutral (DOPC, Egg-PC, POPC, POPE & Egg-PE) and negatively-charged (Liver-PI, Brain-PS, Brain-PI(4,5)P2 & Brain-PI4P) lipid mixtures.
Specifications:
- Surface topographies: round or square pillars, nanodomes or nanocones
- Approx. pattern area: 1 cm x 1 cm
- Surface material: borosilicate glass
- Coverslip diameter: 25 mm
- Coverslip thickness N°: 1.5H (0.170 mm ± 0.005 mm)
- Cell culture treated: No
- Storage: can be stored indefinitely at room temperature when protected from dust & humidity
This FakirSlide Coverslip is manufactured in France by Idylle SAS.
| Size | |
| Sensitivity | |
| Dynamic Range | |
| Incubation Time | |
| Sample Type | |
| Storage | Room Temp |
| Alternative Names | |
Related products
