cDNA Synthesis Kit

cDNA Synthesis Kit

biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases. The combination of highly efficient cDNA synthesis, effective RNase inhibition and pure dNTPs allows high yields of cDNAs of more than 19 kb. For greater application flexibility, hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, for the synthesis of cDNA from only poly(A) tailed mRNA, are included.

This cDNA Synthesis Kit is manufactured in Germany by biotechrabbit.

Size	10 rxn of 20 µL | 125 rxn of 20 µL | 250 rxn of 20 µL
Component	RevertUP II Reverse Transcriptase – RevertUP II Reverse Transcriptase, 200 U/µl in Storage buffer, containing 50% glycerol 5× cDNA Synthesis Buffer – Optimized 5× cDNA synthesis buffer dNTP Mix (10 mM each) – Aqueous solution (pH 7.0) containing 10 mM each: dATP, dCTP, dGTP, dTTP sodium salts RNase Inhibitor – RNase Inhibitor, 40 U/µl, in Storage buffer, containing 50% glycerol Hexamer Primer – 25 µM Random Hexamer Primer Oligo (dT) Primer – 10 µM Oligo (dT) Primer PCR Grade Water – Ultrapure, sterile filtrated water, DNase-, RNase- and protease-free
Application	First-strand cDNA synthesis for RT-PCR and qPCR, Gene expression profiling, RNA labeling and primer extension, cDNA library construction
Hot Start	No
Storage	-20°C
Instructions For Use

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