A-Tailing Kit contains the essential reagents required for the addition of an adenine nucleotide to the 3′ ends of DNA fragments. A-Tailing Kit is for research use only and not to be used for diagnostic procedures.
This kit includes a 20X A-Tailing Mix, a 1 mM dATP solution, and a 10X Activation Buffer. The reaction is designed to be performed in a single step and ensures a highly efficient A-tailing process, with minimal risk of overhang loss. The kit components are optimized to work together, providing consistent results with high specificity.
This A-Tailing Kit is manufactured in Germany by biotechrabbit.
1 mM dATP – Aqueous solution (pH 7.0) of 1 mM 2′-deoxyadenosine 5′-triphosphate sodium salt
10X Activation Buffer – Optimized A-Tailing buffer in 10X concentration
Application
Preparation of DNA for cloning into T/A cloning vectors, Suitable for sequencing applications, Ideal for Next Generation Sequencing (NGS) library preparation, Compatible with a broad range of DNA templates.
