Blog

10 Reasons to Keep Up with Your Vitamin D Levels


We spend so much time trying not to get sun exposure so we can prevent skin cancer only to find out that as a result we are not getting the vitamin D we need.

So what’s the deal? Are we going to become feeble with a lack of vitamin D? It turns out that not getting enough of this super vitamin could lead to several medical conditions/diseases. Read more

In
fact, here are 10 reasons to keep up your vitamin D

levels:

Several Types of Cancers

High Blood Pressure

Heart Disease

Depression

Fibromyalgia

Chronic Muscle Pain

Bone Loss

Diabetes

Other Autoimmune Diseases Such as Multiple Sclerosis                                                          

And last but certainly NOT least…

Alzheimer’s and
Dementi
a

In a recent study, individuals over age 65 had their Vitamin D levels tested and then they were followed for a number of years: 

During
this follow-up period, 171 of the participants developed dementia and 102
participants developed Alzheimer’s disease. The researchers found the
participants with low levels of vitamin D were 53% more likely to develop
dementia, and those who were severely deficient were 125% more likely, when
compared with participants with regular levels of vitamin D.
Read more

This research is intriguing because Alzheimer’s is killing more people each year, in fact even
more than prostrate cancer and breast cancer combined. Recent research in this area has revealed
that this disease is caused by a build up of abnormal proteins such as
beta-amyloids in the brain.This leads
researchers to believe that genetic testing (for APOE) for early intervention
and therapy that will either decrease amyloid-beta production or increase the
degradation of these proteins could be beneficial to these patients. Read more

Alzheimer’s is extremely
underfunded especially when compared to other areas of research.  Scientists are making some head way in this
area but the lack of funding is dramatically hindering the path to a cure.

So we ask all of you…Shall we tackle
this problem like supporters of the ALS foundation did with the Ice Bucket Challenge?

Using the right timing (we might need to let
the ice bucket challenge finish its course) and the right challenge it might
work! Let us know if you have any good
ideas for such a challenge and perhaps this time lets make it involve something other than water! It needs
to be quick and/or funny and powerful! Give us ideas! Ready, Set, Go!

And if you want to donate to the
Alzheimer’s Association
Click here

FGF-21 ELISA Promotion at EagleBio for August 2014

Product Discounts available through the end of August 2014:

Intact FGF-21 ELISA at a 30% discount:

Eagle
Biosciences is pleased to introduce the first Intact FGF-21 ELISA assay kit
that truly measures the active human intact FGF-21 (1-181) with no cross
reaction to any FGF-21 fragments. Other assays that determine fragments of the
FGF-21 can overestimate the biological activity of the protein. The
physiological functions of FGF-21
require intact molecular structure and amino acid sequence in its N-terminal
and C-terminal region.

Use
Code FGF0830 when ordering to take advantage of this discount.

Click here to view the:

FGF-21 ELISA PROMOTION

Looking for work? The Tooth Fairy may be recruiting new talent.
Is it possible that the days of the tooth fairy visiting your house and leaving
your child money are over?! We may have to start paying her for her newly
acquired task. New research is being done to investigate the process of nerve
cells spontaneously forming into stem cells. Read more

This opens doors for a variety of emerging areas of research and therapies such
as Diabetes, spinal cord injuries, and several other diseases/medical conditions. One company has taken
advantage of this opportunity and has started offering a bank to store teeth
for those who may need or worry about needing stem cell therapy in the future. Read more

So I ask you… do we need to leave special instructions under our child’s
pillow for the Tooth Fairy if we want their teeth to be delivered to
Store-a-Tooth to become toothsicles?!

But in all seriousness, this is fascinating research that could lead to amazing medical breakthroughs.

EagleBio Product Discounts Available – July 2014

Product Discounts available through the end of July 2014:

VITAMIN D ELISA:

The Vitamin D ELISA Assay Kit promotion is back for a short time!  A quality assay that measures both Vitamin D2 and Vitamin D3 at 100%.  With excellent correlation to LC/MS, results can be determined in less than 3 hours.

Use Code VID0740 when ordering to receive a 40% discount.  One kit per customer is available at the discounted price.


sLHCGR ELISA KITS:

Newly launched assays for measuring Total sLHCGR, as well as complexed LH-sLHCGR and hCG-sLHCGR are discounted to allow new customers to try one or all of these unique and important ELISA kits.  These new assays will help researchers learn more about the one receptor (LHCGR) and the two hormones (LH and hCG) it binds.  Preliminary studies indicates that these receptors can provide very unique information to fertility researchers that cannot be ascertained from other fertility biomarkers such as AMH.  

Use Code HOR0730 when ordering to receive a 30% discount.  One of each kit per customer is available at the discounted price.

Eagle Biosciences Expands Further into Research Market with Launch of New Website

July 1, 2014: Eagle Biosciences, Inc.
is pleased to announce the re-launch of a newly updated & fully-featured
website, www.eaglebio.com.  The
new site offers extensive scientific content including a new blog feature which
is updated frequently in support of EagleBio promotions and new products.  The website also offers informative Biomarker
Spotlights highlighting key biomarkers within their product catalog and a
new simplified ordering process to make check out quick and efficient.

Dan Keefe, President is excited about the launch
of Eagle Biosciences’ website. “This is a turning point for EagleBio as our new
website not only provides an updated look and feel, but it also truly connects
us to the research community in several ways through the expansion of our
scientific content and social media. The
changes that we have implemented help us to continue to meet our company
objective to quickly provide our customers with as much information as possible
so that they can make efficient, well-informed decisions.” Keefe concluded, “We
expect the website to continually evolve with the intention to provide
tremendous value to our customers.”

Eagle anticipates more changes coming to the
website in order to continue to improve and better meet customers’ needs. In
addition, Eagle Biosciences plans to be launching a quarterly newsletter, Eagle
iView, at the end of July 2014.  Eagle
iView is intended to update customers on new products, new biomarkers, hot
promotions, and interesting research in the field.

EagleBio Biomarker Spotlight:  Homoarginine





What is Homoarginine?

Homoarginine
is a non-essential cationic amino acid, which is formed from lysine and it is a
precursor of nitric oxide (NO).  This
amino acid has been thought to be involved in increasing the availability of NO
which is the basis of its many roles including enhancing endothelial and myocardial
functions.In vitro and in vivo, Homoarginine shows characteristics similar to arginine. Epidemiological
investigations in two large independent cohorts, namely the German diabetes
dialysis (4D)  study and the Ludwigshafen Risk and Cardiovascular Health
(LURIC)  study have identified homoarginine as useful predictor of cardiovascular
events and mortality.

In other recent
studies, a correlation of increasing homoarginine levels to increasing age was
noted when observing healthy children and adolescents. This age-dependent increase in plasma
concentration is theorized that it is caused as a result of gradual maturation
of the pathways controlling homoarginine metabolism.  Despite these results, homoarginine and
ornithine do not appear to be linked with carotid vascular structure in healthy
children and adolescents.

Beyond
cardiovascular implications, homoarginine concentrations have also been found
to be directly correlated with kidney function and are significantly associated
with the progression of chronic kidney disease (CKD).
Low homoarginine
concentrations might be an early indicator of kidney failure and a potential
target for the prevention of disease progression but this theory needs further
exploration and investigation. The
results from Ravani et al. reveal that homoarginine could be a useful marker
for monitoring hemodialysis patients.  In
fact, these results may provide the foundation and basis of designing such clinical
trials. 


Why Measure Homoarginine?

Homoarginine
as described above has many roles and potential effects on various functions
and organs in the body as it has been demonstrated from numerous studies that
it has implications in endothelial, cardiovascular, and renal events. Other specific areas of research that
homoarginine metabolism have been linked to are ischemic strokes and pregnancy
pathologies such as preeclampsia.This analyte continues to be the focus of numerous studies and fields of
research.


Related Kits:

Homoarginine ELISA Assay kit

Cardiovascular Assay Kits


References:

  1. Choe, CU et al. “Homoarginine levels are regulated by
    L-arginine: glycine amidinotransferase and affect outcome: results from
    human and murine studies.” Circulation 2013; 128 (13): 1451-61. https://www.ncbi.nlm.nih.gov/pubmed/24004504
  2. Drechler, Christiane et al. “Homoarginine and
    Progression of Chronic Kidney Disney: Results from the Mild to Moderate
    Kidney Disease Study.” PLOS One 2013; DOI: 10.1371/journal.pone.0063560. https://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063560#s1
  3. Jazwinska-Kozuba et al. “Opposite Associations of
    Plasma Homoarginine and Ornithine with Arginine November 2013; 14,
    21819-21832in Healthy Children and Adolescents”. International Journal of
    Molecular Sciences. https://www.mdpi.com/1422-0067/14/11/21819
  4. Khalil, AA et al. “Asymmetric dimethylarginine,
    arginine and Homoarginine at 11-13 weeks’ gestation and preeclampsia: a
    case-control study.” Journal of Human Hypertension, 2013; 27, 38-43. https://www.readcube.com/articles/10.1038/jhh.2011.109
  5. Marz, Winfried et al. “Homoarginine, Cardiovascular
    Risk, and Mortality.” Circulation , 2010; 112: 967-975. https://circ.ahajournals.org/content/122/10/967.long
  6. Meinitzer, Andreas et al. “Homoargine: a new
    cardiovascular risk marker in hemodialysis patients.” Journal of
    Laboratory Medicine 2011; 35(3): 153-159. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3143829/
  7. Ravani, Pietro et al. “Homoarginine and Mortality in
    Pre-Dialysis in Chronic Kidney Disease (CKD) Patients.”PLOS One 2013;
    DOI: 10.1371/journal.pone.0072694.g003. https://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0072694

EagleBio Biomarker Spotlight:  ADMA




What is ADMA?

ADMA the abbreviation for asymmetric dimethylarginine and it is endogenous molecule which can be detected in human blood and urine. It shows structural homology to the amino acid L-arginine, and it acts as an inhibitor of nitric oxide (NO) synthesis. (5,6)

In fact, various studies performed in vitro and in vivo have confirmed and demonstrated that ADMA concentration-dependently inhibits NO production.(3,5)  Böger RH et al. 2004 study discusses the mechanism of the modulation of NOS activity. This study describes how the inhibition of the enzyme that inactivates ADMA, dimethylarginine dimethylaminohydrolase (DDAH), thereby elevating ADMA levels and causing vasoconstriction of isolated arterial rings in vitro. (2)  DDAH’s role in degradation of ADMA is the major pathway for its elimination however, a small amount is eliminated by renal excretion. (4)

Pathophysiological Role of ADMA
The vascular endothelium plays a central role in the regulation of vascular structure and function, mainly due to the formation of endothelium-derived nitric oxide (NO). NO has been named an “endogenous anti-atherogenic molecule” due to its diverse regulatory functions in vascular homeostasis. In fact, ADMA has been found to cause vasoconstriction when it is infused intraarterially. NO is formed by the enzyme NO synthetase (NOS) from the amino acid precursor L-arginine and it plays a large role within cardiovascular system. NOS activity can be down-regulated by asymmetric dimethylarginine (ADMA), an endogenous inhibitor of NOS as described above. (3,5,6)


In past studies, patients with end-stage chronic renal failure and have little or no urine output, elimination is thus blocked and as a result circulating concentrations of ADMA rise appropriately to inhibit NO synthesis. The accumulation of ADMA is thought to lead to impaired NOS synthesis as described above this is theorized to be a contributing factor to hypertension and immune dysfunction associated with chronic renal failure. (4)  In addition to ADMA’s effects on vasodilation, a rise in circulating ADMA levels lead to an increased resting vascular tone. It also enhances several pro-atherogenic mechanisms including platelet aggregation and adherence of monocytes, proliferation of vascular smooth muscle cell, as well as extracellular matrix formation. (4)


The effects of ADMA on NO synthesis and NO-mediated pathophysiological processes have been described in numerous experimental studies. Moreover, elevated ADMA levels in plasma have been found in clinical studies including patients with hypercholesterolemia, hypertension, chronic heart failure, chronic renal failure and other internal disorders. Recent prospective and cross-sectional studies indicated that elevated ADMA levels are a risk factor for future cardiovascular eve
nts and total mortality. ADMA may have diagnostic relevance as a novel cardiovascular risk marker. (2,3)



Diseases Associated with Elevated ADMA Levels:

  • Coronary Artery Disease
  • Chronic Renal Failure and Hemodialysis Treatment
  • Peripheral Arterial Occlusive Disease
  • Chronic Heart Failure
  • Hypertensive patients
  • Diabetes Mellitus
  • Lipid Disorders
  • Preeclampsia
  • Erectile Dysfunction


What Methods can be used to Measure ADMA?

Eagle Biosciences offers many options for researchers looking to measure ADMA:


References:

  1. Antoniades,
    C et al. “ Asymmetrical dimethylarginine regulates endothelial function in
    methionine-induced but not in chronic homocystinemia in humans: effect of
    oxidative stress and proinflammatory cytokines.”
    Am. J. Clin. Nutr. 2006; 84: 781-788. https://ajcn.nutrition.org/content/84/4/781.full
  2. Böger
    RH et al. “Asymmetric Dimethylarginine, an Endogenous Inhibitor of Nitric Oxide
    Synthase, Explains the “l-Arginine Paradox” and Acts as a Novel Cardiovascular
    Risk Factor.”
    J. Nutr. 2004; 134 no. 10 2842S-2847S.https://jn.nutrition.org/content/134/10/2842S.long
  3. Böger
    RH et al. “Asymmetric dimethylarginine: a novel risk factor for endothelial
    dysfunction. Its role in hypercholesterolemia.”
    Circulation 1998; 98: 1842 – 1847.https://cardiovascres.oxfordjournals.org/content/59/4/824.full.pdf+html
  4. Krempl
    TK et al. “Elevation of asymmetric dimethylarginine (ADMA) in patients with
    unstable angina and recurrent cardiovascular events.” Eur. Heart J. 2005; 26:
    1846-1851.
    https://eurheartj.oxfordjournals.org/content/26/18/1846.full
  5. Vallance
    P. et al. “ Accumulation of an endogenous inhibitor of NO synthesis in chronic
    renal failure”.
    Lancet 1992; 339: 572
    – 575.
    https://www.ncbi.nlm.nih.gov/pubmed/1347093?dopt=Abstract
  6. Vallance
    P. et al. “ Endogenous dimethyl-arginine as an inhibitor of nitric oxide
    synthesis”.
    J. Cardiovasc. Pharmacol.
    1992; 20 (Suppl. 12): S60 – S62.
    https://jn.nutrition.org/content/134/10/2842S.long


For a Full Listing of Publications utilizing these ADMA ELISA Assay kits, click on the link below:

ADMA ELISA Assay Kit Publications

Related News

Eagle Biosciences introduces new ultrasensitive Steroid Saliva ELISA kits that will allow for quantification of many samples that were previously undetectable with a traditional steroid saliva assay. The list of new immunoassays includes Ultrasensitive Salivary Cortisol ELISA and Ultrasensitive Salivary DHEA-S ELISA with Ultrasensitive Salivary Testosterone ELISA, Ultrasensitive Salivary Progesterone ELISA, and Ultrasensitive Salivary Estradiol ELISA coming soon.

Dan Keefe, President of Eagle, is excited about the new products, “We are thrilled to offer the new assays as they could change the way researchers measure salivary Cortisol, Testosterone, Progesterone, Estradiol, and DHEA-S. In addition to their sensitivity, the steroid kits are easy to run and will provide results in less than 3 hours.”

For more information on these kits or any of the products from Eagle Biosciences, visit our website at www.EagleBio.com or call us at 617-419-2019.

Eagle Biosciences, Inc. is proud to introduce a new Mouse Monoclonal Antibody Isotyping ELISA kit. The Mouse Monoclonal Antibody (mAb) Isotyping assay kit is designed for the quick and easy identification of both mouse immunoglobulin subclass and light chain. Utilizing a simple 96 well (8×12) ELISA format, up to 12 individual samples can be determined per 96 strip-well plate for heavy chain anti-IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM as well as light chain kappa and lambda. One sample is used per strip utilizing a traditional ELISA plate reader.

“This ELISA kit has excellent specificity for each subtype and results can be produced in about a hour while keeping costs per sample below $6.”said Dan Keefe, President of Eagle,”This product is just the tip of the iceberg for Eagle. We are thrilled about this product launch because it represents the first of a line of Eagle developed assays that will isotype individual mouse monoclonal antibody subclasses.” Eagle expects to introduce individual ELISA based assays for the mouse monoclonal antibody isotypes as soon as this summer.”

The Eagle Mouse Monoclonal Antibody Isotyping ELISA kit is available in convenient 1 plate, 5 plate, or custom bulk sizes and is priced very competitively. For more information on this kit or any of the products from Eagle Biosciences, visit the website at www.EagleBio.com or call at 866-411-8023.